科學研究：

研究方向：

主要從事工業(yè)酶基因的選殖、酶蛋白純化、基因改造、酶學研究（包含熱力學及動力學）、X光蛋白晶體學、膜蛋白三度空間結構之解析、工業(yè)酶及膜蛋白發(fā)酵技術探究。

承擔科研項目情況：

1． 科技支撐計劃項目：新型紡織酶的X光結構解析、基因改造及開發(fā)，負責人。

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科研成果：

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論文專著：

在 PNAS， JBC，Structure，JACS，JMB，Proteins 等雜志上發(fā)表 SCI 論文共20余篇。

出版專著：

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發(fā)表論文：

1. Guo, R. T., Chou, L. J., Chen, Y. C., Chen, C. Y., Pari, K, Jen, C. Y., Lo, S. C., Huang, S. L., Lee, C. Y., Chang, T. W., Chuang, W. J. (2001) Expression in Pichia pastoris and Characterization by Circular and NMR of Rhodostomin. Proteins: Structure, Function, and Genetics. 43, 499-508.

2. Guo, R. T., Ko, T. P., Chou, C. C., Shr, H. L., Chu, H. M., Tsai, Y. H., Liang, P. H., and Wang, A. H.-J. (2003) Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli. Acta Crystallogr. Sect. D Biol. Crystallogr. 59, 2265-2268.

3. Guo, R. T., Kuo, C. J., Chou, C. C., Ko, T. P., Shr, H. L., Liang, P. H., and Wang, A. H.-J. (2004) Crystal structure of octaprenyl pyrophosphate synthase from hyperthermophilic Thermotoga maritima and mechanism of product chain length determination. J. Biol. Chem. 279, 4903-4912.

4. Guo, R. T., Kuo, C. J., Ko, T. P., Chou, C. C., Liang, P. H., and Wang, A. H.-J. (2004) A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long-chain trans-prenyl products. Biochemistry. 43, 7678-7686.

5. Chu, H. M., Guo, R. T., Lin, T. W., Chou, C. C., Shr, H. L., Lai, H. L., Tang, T. Y., Cheng, K. J. Selinger, B. L., and Wang, A. H.-J. (2004) Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis. Structure. 12, 2015-2024.

6. Guo, R. T., Ko, T. P., Chen, A. P.-C. Kuo, C. J., Wang, A. H.-J., and Liang, P. H. (2005) Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate and farnesyl thiopyrophosphate: Roles of the metal ion and conserved residues in catalysis. J. Biol. Chem. 280, 20762-20774.

7. Sun, H. Y., Ko, T. P., Kuo, C. J., Guo, R. T., Chou, C. C., Liang, P. H., and Wang, A. H.-J. (2005) Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures. J. Bacteriol. 187, 8137-8148.

8. Chang, T. H., Guo, R. T., Ko, T. P., Wang, A. H.-J., and Liang, P. H. (2006) Crystal structure of type-III geranylgeranyl pyrophosphate synthase from Saccharomyces cerevisiae and the mechanism of product chain length determination. J. Biol. Chem. 281, 14991-15000.

9. Guo, R. T., Cao, R., Ko, T. P., Chang, T. H., Jeng, W. Y., Kuo, C. J., Chen, C. K.-M., Hudock, M. P., Zhang, Y., Song, Y., Liang, P. H., Oldfield, E., and Wang, A. H.-J. (2007) Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases. Proc. Natl. Acad. Sci. USA 104, 10022-10027.

10. Jeng, W. Y., Ko, T. P., Liu, C. I., Guo, R. T., Liu, C. L., Shr, H. L., and Wang, A. H.-J. (2008) Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis. Nucleic Acids Res. 36, 1567-77.

11. Kuo, C. J., Guo, R. T., Lu, I. L., Liu, H. G., Wu, S. Y., Ko, T. P., Wang, A. H.-J., and Liang, P. H. (2008) Structure-based inhibitors exhibit differential activities against Helicobacter pylori and Escherichia coli undecaprenyl pyrophosphate synthases. J Biomed Biotechnol. 2008, 841312.

12. Cao, R., Chen, C. K.-M., Guo, R. T., Wang, A. H.-J., and Oldfield, E. (2008) Structures of a potent phenylalkyl bisphosphonate inhibitor bound to farnesyl and geranylgeranyl diphosphate synthases. Proteins: Structure, Function, and Genetics. 73, 431-9.

13. Chen, C. K.-M., Hudock, M. P., Zhang, Y., Guo, R. T., Cao, R., No, J. H., Liang, P. H., Ko, T. P., Chang, T. H., Chang, S. C., Song Y. C., Axelson, J., Kumar, A., Wang, A. H.-J., and Oldfield, E. (2008) Inhibition of Geranylgeranyl Diphosphate Synthase by Bisphosphonates: A Crystallographic and Computational Investigation. Journal of Medicinal Chemistry. 51, 5594-607.

14. Wu, Y.H., Ko, T.P., Guo, R.T., Hu, S.M., Chuang, L.M., Wang, A.H.-J. (2008) Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2. Structure. 16, 1714-23.

15. Zhang, Y., Cao, R., Yin, F., Hudock, M., Guo, R.T., Krysiak, K., Mukherjee, S., Gao, Y.G., Robinson, H., Song, Y., No, J.H., Bergan, K., Leon, A., Cass, L., Goddard, A., Chang, T.K., Lin, F.Y., van, B.E., Papapoulos, S., Wang, A.H.-J, Kubo, T., Ochi, M., Mukkamala, D., Oldfield, E. (2009) Lipophilic Bisphosphonates as Dual Farnesyl/Geranylgeranyl Diphosphate Synthase Inhibitors: An X-ray and NMR Investigation . JACS 131, 5153-62.

16. Chen, C.K.-M., Lee, G.C., Ko, T.P., Guo, R.T., Huang, L.M., Liu, H.J., Ho, Y.F., Shaw, J.F., Wang, A.H.-J. (2009) Structure of the Alkalohyperthermophilic Archaeoglobus fulgidus Lipase Contains a Unique C-Terminal Domain Essential for Long Chain Substrate Binding. Journal of Molecular Biology. 390, 672-85.

17. Guo, R.T., Chong, Y.E., Guo, M., Yang, X.L. (2009) Unique mechanism for Ap4A synthesis by human Glycyl-tRNA synthetase. J. Biol. Chem. 284, 28968-76.

18. Cheng, Y.S., Ko, T.P., Wu, T.H., Ma, Y., Huang, C.H., Lai, H.L., Wang, A.H.-J., Liu, J.R.,* and Guo, R.T.* (2011) Crystal structure and substrate-binding mechanism of cellulase 12A from Thermotoga maritima. Proteins: Structure, Function, and Bioinformatics.79, 1193-204. (featured on cover)

19. Zeng, Y.F., Ko, T.P., Lai, H.L., Cheng, Y.S., Wu, T.H., Ma, Y., Chen, C.C., Yang, C.S., Cheng, K.J., Huang, C.H., Guo, R.T.* and Liu, J.R.* (2011) Crystal structures of Bacillus alkaline phytase in complex with calcium and inositol hexasulfate. Journal of Molecular Biology 409, 214-24. (featured on cover)

20. Wu, T.H., Huang, C.H., Ko, T.P., Lai, H.L., Ma, Y., Chen, C.C., Cheng, Y.S., Liu, J.R.,* and Guo, R.T.* (2011, in press) Diverse Substrates Recognition Mechanism Revealed by Thermotoga maritima Cel5A Structures in Complex with Cellobiose and Mannotriose. BBA-Proteins and Proteomics. (featured on cover)

21. Tseng, C.W., Ko, T.P., Guo, R.T., Huang, J.W., Wang, H.C., Huang, C.H., Cheng, Y.S., Wang, A.H.-J., and Liu, J.R. (2011, in press) Substrate binding of a GH5 endoglucanase from the ruminal fungus Piromyces rhizinflata. Acta Crystallographic Section F. 67, 1189-1195. (featured on cover)

22. Huang, J.W., Cheng, Y.S., Ko, T.P., Lin, C.Y., Lai, H.L., Chen, C.C., Ma, Y., Zheng, Y., Huang, C.H., Zou, P., Liu, J.R.,* and Guo, R.T.* (2011, in press) Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-β-D-glucanase. Appl Microbiol Biotechnol.

榮譽獎勵：

1． 2007年獲中國化學制藥股份有限公司第十七屆王民寧先生獎之國內醫(yī)藥研究所博士班優(yōu)秀論文獎第一名。

2． 2007年獲臺大生命科學院博士畢業(yè)生院長獎第一名。

3． 2007年獲臺灣行政院國家科學委員會補助赴國外從事博士后研究之出國留學獎學金。

4． 2010年入選天津千人計劃。

5． 2011年入選中國科學院“百人計劃”。

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